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Protocol
[Santa Cruz] Flow Cytometry | ||
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Flow CytometryA. Sample PreparationPrepare cells according to cell type. BLOOD(Human, Mouse or Rat)
Mouse Spleen or Other Tissue
Suspension Cell Line
Monolayer / Adherent Cell Line
B. CELL STIMULATIONStimulate cells as necessary. C. STAIN PREPARATIONFix cells or prepare live cells for staining. NOTE: It may be desired to block Fc receptors for certain cell types including, but not limited to, mouse and rat blood, mouse spleen, mouse bone marrow, etc. A blocking reagent contains a high concentration of immunoglobulin that will bind to the Fc-receptors on cells. The blocking reagent should ideally be immunoglobulin from the species whose cells you are staining. Recommended blocking reagent: 1 µg normal immunoglobulins per 1 million cells. Live Staining
Fixed and Permeabilized Cells for Intracellular Staining
NOTE: You should only proceed with permeabilization if you can stain immediately afterwards.
NOTE: Important: If a pellet is not recovered at this step, be sure to spin again and try to recover more cells.
D. STAININGFollow protocol for direct or indirect staining. DIRECT STAINING(With Fluorochrome - Conjugated Antibodies)
INDIRECT STAINING(With Unconjugated Primary Antibodies and Fluorochrome - Conjugated Mouse IgG Binding Proteins or Secondary Antibodies)
E. ACQUIREAcquire within 24 hours. |
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관리자 | DATE 2022-11-24 11:05:13 | |