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Protocol
[Santa Cruz] Immunoperoxidase Staining | ||
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Immunoperoxidase StainingA. Tissue Culture CellsGrow cultured cells in chambered culture slides overnight at
Optional: Incubate for 5–7 minutes in 0.1–1% hydrogen peroxide in PBS to quench endogenous peroxidase activity. Wash in PBS twice for 5–7 minutes each. B. Frozen Tissue Sections
Optional: Incubate for 5–7 minutes in 0.1–1% hydrogen peroxide in PBS to quench endogenous peroxidase activity. Wash in PBS twice for 5–7 minutes each. NOTE: For tissues containing high levels of endogenous biotin (which may result in higher background staining), we recommend following the Formalin-Fixed, Paraffin-Embedded Tissue Sections protocol, as endogenous biotin is normally destroyed in paraffin-embedded tissue. C. Formalin-Fixed, Paraffin-Embedded Tissue Sections
NOTE: Alternatively, other non-xylenes clearing agents can be used for tissue deparaffinization (e.g. Histo-Clear). Optional: Antigen unmasking may be performed at this point. Certain antigenic determinants are masked by formalin fixation and paraffin embedding and may be exposed by one of several methods:
Optional: Incubate for 5–7 minutes in 0.1–1% hydrogen peroxide in deionized H2O to quench endogenous peroxidase activity. Wash in PBS twice for 5–7 minutes each. D. Indirect Immunoperoxidase Staining
ABC Staining Protocol
Optional: Counterstain slides in Gill's Hematoxylin Solution, No. 2 (sc-24973) for 5–10 seconds and immediately wash with several changes of deionized H2O.
NOTE: For a listing of mounting media for immunohistochemistry, including Organo/Limonene (sc-45087) and ImmunoHistoMount (sc-45086) for immunoperoxidase staining, please browse the Mounting Media for Staining. LSAB Staining Protocol
Indirect Immunoperoxidase Staining Using Mouse IgG Binding Proteins
E. Direct Immunoperoxidase Staining
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관리자 | DATE 2022-07-08 09:46:52 | |